The cell's membrane is not large enough to accommodate the excess fluid, causing the membrane to break open, or lyse. Maintaining an osmotic balance is a very basic but very important function of the cell membrane to prevent cytolysis. Dosage of plaquenil Valtrex and plaquenil Hydroxychloroquine bleaching cream Plaquenil pill shape Osmotic lysis is the bursting of a cell, aka a "cell explosion" or "cytolysis", because of an overabundance of fluid. The cell's membrane is not large enough to accommodate the excess fluid, causing the membrane to break open, or lyse. Ca 2+ may induce endosomal escape via the proton sponge effect, which works by causing osmotic swelling and eventual rupture of the vesicles 11,17–20. Similarly, chloroquine can induce the vesicle rupture through protonation in acidic environment of late endosomes and lysosomes 11. Osmotic lysis of pinosomes has already been used with a number of cultured cell lines to study intracellular proteolysis, cytoplasmic pH changes, Ca 2+ movements, and uptake of toxins, and to introduce antibodies into the cytoplasm for the studies on microtubule function. Rates of pinocytosis are under physiological control, and they can be manipulated by growth conditions and various chemicals. Cells are the basic functional unit of the body and of life. Most of the things that cells do depend on the flow of certain ions into and out of the cell. Chloroquine and osmotic lysis of endosome Endosome-to-Plasma Membrane Recycling of VEGFR2 Receptor., Distinct effects of endosomal escape and inhibition of. Hydroxychloroquine painkiller The Toll-like receptors TLRs are key initiators of innate and adaptive immune responses through production of proinflammatory cytokines and chemokines, upregulation of costimulatory molecules and activation of antigen presentation Akira and Takeda, 2004. Endocytic pathways regulate Toll-like receptor 4 signaling and link.. Osmotic lysis of pinosomes - ScienceDirect. Inhibition of Endosomal/Lysosomal Degradation Increases.. As mentioned before, calcium phosphate is acid-sensitive, and its degradation in endosome/lysosome increases osmotic pressure of endosome, leading to release of siRNA into the cytoplasm. In addition, lipid component is well known to be helpful for nanoparticles uptake and cargo release from endosome. Dec 22, 2006 Effect of chloroquine treatment on the levels of endosome-binding proteins in PC12 cells. PC12 cells were treated with 1 μM chloroquine for 5 days. A Cell lysates were subjected to Western blotting using CTB-HRP and specific antibodies. B The intensities of the bands were determined by quantitative densitoscanning. Chloroquine is effective in preventing the spread of SARS CoV in cell culture. Favorable inhibition of virus spread was observed when the cells were either treated with chloroquine prior to or after SARS CoV infection.